Please paste one or more FASTA-formatted sequences in the textarea below:

Select mode: MSA-version (1 sequence)   Single-sequence version (up to 20000 sequences)

Select algorithm to use: HMM (original version)   HMM + dipeptides   HNN

Run signal-peptide prediction using PRED-TAT:

Use library of characteristic pHMMs for β-barrel regions derived from OMPdb in prediction:

Prior information (constrained prediction):

Enter your email address to get notification of results:

Note: If you wish to perform a batch submission of more than 20000 sequences, please contact kostas.tsirigosgmail.com directly.

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- If you find PRED-TMBB2 useful in your research, please cite the reference that describes this work:
PRED-TMBB2: Improved topology prediction and detection of beta-barrel outer membrane proteins
PMID: 27587687

- If you used PRED-TMBB2 with the dipepetide mode, please also cite the reference that describes this work:
Extending hidden Markov models to allow conditioning on previous observations
PMID: 30353782

- If you used PRED-TMBB2 + HNN, please also cite the reference that describes this work:
Hidden Neural Networks for biological sequence analysis
PMID: 34849210

- PRED-TMBB2 can be run locally, using the JUCHMME Java package:
JUCHMME: a Java Utility for Class Hidden Markov Models and Extensions for biological sequence analysis.
PMID: 31250907

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Download the training dataset here
Download the discrimination dataset here